RESUMO
Hantaviruses are important contributors to disease burden in the New World, yet many aspects of their distribution and dynamics remain uncharacterized. To examine the patterns and processes that influence the diversity and geographic distribution of hantaviruses in South America, we performed genetic and phylogeographic analyses of all available South American hantavirus sequences. We sequenced multiple novel and previously described viruses (Anajatuba, Laguna Negra-like, two genotypes of Castelo dos Sonhos, and two genotypes of Rio Mamore) from Brazilian Oligoryzomys rodents and hantavirus pulmonary syndrome cases and identified a previously uncharacterized species of Oligoryzomys associated with a new genotype of Rio Mamore virus. Our analysis indicates that the majority of South American hantaviruses fall into three phylogenetic clades, corresponding to Andes and Andes-like viruses, Laguna Negra and Laguna Negra-like viruses, and Rio Mamore and Rio Mamore-like viruses. In addition, the dynamics and distribution of these viruses appear to be shaped by both the geographic proximity and phylogenetic relatedness of their rodent hosts. The current system of nomenclature used in the hantavirus community is a significant impediment to understanding the ecology and evolutionary history of hantaviruses; here, we suggest strict adherence to a modified taxonomic system, with species and strain designations resembling the numerical system of the enterovirus genus.
Assuntos
Orthohantavírus/classificação , Geografia , Orthohantavírus/isolamento & purificação , Humanos , Filogenia , América do Sul , Especificidade da EspécieRESUMO
We associated Laguna Negra virus with hantavirus pulmonary syndrome in Mato Grosso State, Brazil, and a previously unidentified potential host, the Calomys callidus rodent. Genetic testing revealed homologous sequencing in specimens from 20 humans and 8 mice. Further epidemiologic studies may lead to control of HPS in Mato Grosso State.
Assuntos
Síndrome Pulmonar por Hantavirus/veterinária , Orthohantavírus/genética , Doenças dos Roedores/virologia , Animais , Arvicolinae , Teorema de Bayes , Brasil/epidemiologia , Variação Genética , Orthohantavírus/classificação , Síndrome Pulmonar por Hantavirus/epidemiologia , Síndrome Pulmonar por Hantavirus/virologia , Humanos , Funções Verossimilhança , Camundongos , Tipagem de Sequências Multilocus , Proteínas do Nucleocapsídeo/genética , Filogenia , Doenças dos Roedores/epidemiologiaRESUMO
To study the dynamics of wild rodent populations and identify potential hosts for hantavirus, we conducted an eco-epidemiologic study in Campo Novo do Parecis, Mato Grosso State, Brazil. We detected and genetically characterized Castelo dos Sonhos virus found in a species of pygmy rice rat (Oligoryzomys utiaritensis).
Assuntos
Reservatórios de Doenças/veterinária , Infecções por Hantavirus/veterinária , Orthohantavírus/genética , Doenças dos Roedores/epidemiologia , Sigmodontinae/virologia , Animais , Anticorpos Antivirais/sangue , Brasil/epidemiologia , DNA Viral/análise , Reservatórios de Doenças/virologia , Orthohantavírus/imunologia , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/virologia , Filogenia , Doenças dos Roedores/virologia , Análise de Sequência de DNARESUMO
To confirm circulation of Anajatuba virus in Maranhao, Brazil, we conducted a serologic survey (immunoglobulin G ELISA) and phylogenetic studies (nucleocapsid gene sequences) of hantaviruses from wild rodents and persons with hantavirus pulmonary syndrome. This virus is transmitted by Oligoryzomys fornesi rodents and is responsible for hantavirus pulmonary syndrome in this region.
Assuntos
Reservatórios de Doenças/virologia , Monitoramento Ambiental , Síndrome Pulmonar por Hantavirus/epidemiologia , Orthohantavírus/classificação , Sigmodontinae/virologia , Adulto , Animais , Anticorpos Antivirais/sangue , Brasil/epidemiologia , Busca de Comunicante , Estudos Transversais , Monitoramento Epidemiológico , Feminino , Orthohantavírus/genética , Orthohantavírus/isolamento & purificação , Síndrome Pulmonar por Hantavirus/sangue , Síndrome Pulmonar por Hantavirus/veterinária , Humanos , Imunoglobulina G/sangue , Masculino , Dados de Sequência Molecular , Filogenia , RNA Viral/análise , RNA Viral/genética , Estudos SoroepidemiológicosRESUMO
We describe evidence of circulation of hantaviruses in the influence area of the Santarém-Cuiabá Highway (BR-163) in the Brazilian Amazon through the prevalence of specific antibodies against hantaviruses in inhabitants living in four municipalities of this area: Novo Progresso (2.16%) and Trairão (4.37%), in state of Pará (PA), and Gua-rantã do Norte (4.74%) and Marcelândia (9.43%), in state of Mato Grosso. We also demonstrate the ongoing association between Castelo dos Sonhos virus (CASV) and hantavirus pulmonary syndrome (HPS) cases in the Castelo dos Sonhos district (municipality of Altamira, PA) and the first report of CASV in the municipalities of Novo Progresso and Guarantã do Norte. The results of this work highlight the risk for a possible increase in the number of HPS cases and the emergence of new hantavirus lineages associated with deforestation in this Amazonian area after the conclusion of paving works on BR-163 Highway.
Assuntos
Anticorpos Antivirais/sangue , Infecções por Hantavirus/virologia , Orthohantavírus/isolamento & purificação , RNA Viral/genética , Adolescente , Adulto , Idoso , Brasil , Criança , Pré-Escolar , Feminino , Orthohantavírus/classificação , Orthohantavírus/genética , Infecções por Hantavirus/diagnóstico , Infecções por Hantavirus/epidemiologia , Síndrome Pulmonar por Hantavirus/virologia , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Filogenia , Reação em Cadeia da Polimerase , Estações do Ano , Estudos Soroepidemiológicos , Adulto JovemRESUMO
We describe evidence of circulation of hantaviruses in the influence area of the Santarém-Cuiabá Highway (BR-163) in the Brazilian Amazon through the prevalence of specific antibodies against hantaviruses in inhabitants living in four municipalities of this area: Novo Progresso (2.16 percent) and Trairão (4.37 percent), in state of Pará (PA), and Gua-rantã do Norte (4.74 percent) and Marcelândia (9.43 percent), in state of Mato Grosso. We also demonstrate the ongoing association between Castelo dos Sonhos virus (CASV) and hantavirus pulmonary syndrome (HPS) cases in the Castelo dos Sonhos district (municipality of Altamira, PA) and the first report of CASV in the municipalities of Novo Progresso and Guarantã do Norte. The results of this work highlight the risk for a possible increase in the number of HPS cases and the emergence of new hantavirus lineages associated with deforestation in this Amazonian area after the conclusion of paving works on BR-163 Highway.
